Research Article

Liposome Model Systems to Study the Endosomal Escape of Cell-Penetrating Peptides: Transport across Phospholipid Membranes Induced by a Proton Gradient

Figure 1

Scheme for the detergent-mediated reconstitution of BR into LUVs (after [11]). Stage I–III: Gradual addition of detergent to LUVs. For optimal reconstitution efficiency, BR should be added during stage II. Detergent is removed by Bio-Beads, and the result is an LUV with incorporated BR.
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