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Journal of Drug Delivery
Volume 2012, Article ID 436710, 4 pages
http://dx.doi.org/10.1155/2012/436710
Research Article

In Vitro Investigation of a Terbinafine Impregnated Subcutaneous Implant for Veterinary Use

1Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine University of Tennessee, Knoxville, TN 37996, USA
2Melatek, LLC, 4738 Bergamot Way, Middleton, WI 53562, USA
3Seimens Molecular Imaging, 810 Innovation Drive, Knoxville, TN 37932, USA

Received 25 January 2012; Accepted 7 June 2012

Academic Editor: Jia You Fang

Copyright © 2012 M. J. Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A terbinafine impregnated subcutaneous implant was evaluated to determine if drug was released into isotonic saline over the course of 6 months at two different temperatures, 37°C and 4°C. These temperatures were chosen to simulate the nonhibernating (37°C) and hibernating body (4°C) temperatures of little brown bats (Myotis lucifugus). Insectivorous bats of North America, including little brown bats, have been devastated by white nose syndrome, a fungal infection caused by Geomyces destructans. No treatments exist for bats infected with G. destructans. Implants were placed into isotonic saline; samples were collected once per week and analyzed with HPLC to determine terbinafine concentrations. The mean amount of terbinafine released weekly across the 28 weeks was approximately 1.7 μg at 4°C and 4.3 μg at 37°C. Although significant differences in the amount released did occur at some time points, these differences were not consistently greater or less at either of the temperatures. This study showed that terbinafine was released from an impregnated implant over the course of 6 months at concentrations ranging from 0.02 to 0.06 μg/mL depending on temperature, which may be appropriate for little brown bats (Myotis lucifugus) infected with Geomyces destructans, the etiologic agent of white nose syndrome.