Native and Complexed IGF-1: Biodistribution and Pharmacokinetics in Infantile Neuronal Ceroid Lipofuscinosis

Table 1

Biodistribution of unbound IGF-1, IGF-1/IGFBP-3, IGFBP-3, and IGF1/NP complex 20 min, 120 min, and 240 min post-i.v. injection in CLN1-/- mice. The activity in all organs and tissue samples is expressed as percentage of injected dose/tissue sample weight (%ID/g). All data are expressed as mean ± standard error of the mean (S.E.M).

Organ	IGF-1 complex
	IGF-1	IGF-1/IGFBP-3	IGFBP-3	IGF-1/NP
20 min
Blood	10.21 ± 0.92	15.69 ± 0.90	12.24 ± 0.49	8.66 ± 0.04
Heart	5.35 ± 0.49	7.70 ± 0.23	4.29 ± 0.55	2.45 ± 0.06
Lung	11.37 ± 1.00	7.81 ± 0.24	10.30 ± 1.95	9.00 ± 0.62
Kidney	148.3 ± 34.48	124.2 ± 9.90	34.98 ± 8.90	42.96 ± 3.83
Liver	5.52 ± 0.50	50.06 ± 2.21	61.19 ± 16.80	56.45 ± 8.44
Spleen	6.22 ± 0.39	28.37 ± 1.22	25.50 ± 13.71	47.15 ± 4.11
Ovary	12.71 ± 1.46	4.22 ± 0.57	3.92 ± 0.52	3.09 ± 0.45
Muscle	2.25 ± 0.24	0.77 ± 0.05	0.73 ± 0.06	0.85 ± 0.00
Brain	0.60 ± 0.04	0.48 ± 0.04	0.42 ± 0.00	0.30 ± 0.00
120 min
Blood	8.01 ± 0.92	10.05 ± 2.66	15.82 ± 2.01	6.30 ± 0.21
Heart	2.95 ± 0.45	3.76 ± 1.22	5.50 ± 0.50	1.71 ± 0.08
Lung	6.57 ± 0.97	7.35 ± 2.42	9.49 ± 1.06	6.37 ± 0.57
Kidney	14.32 ± 2.23	10.31 ± 4.21	12.40 ± 1.19	10.27 ± 1.97
Liver	5.31 ± 0.74	7.95 ± 4.78	8.45 ± 1.28	17.75 ± 0.83
Spleen	5.30 ± 0.56	9.45 ± 3.28	9.40 ± 3.28	14.71 ± 2.49
Ovary	7.04 ± 1.66	7.78 ± 2.14	8.27 ± 0.55	2.99 ± 0.11
Muscle	1.74 ± 0.43	1.75 ± 0.48	1.67 ± 0.07	0.91 ± 0.03
Brain	0.35 ± 0.08	0.40 ± 0.16	0.51 ± 0.04	0.24 ± 0.01
240 min
Blood	5.71 ± 0.41	4.68 ± 0.17	6.24 ± 0.43	4.15 ± 0.39
Heart	1.89 ± 0.15	1.46 ± 0.08	2.03 ± 0.13	1.00 ± 0.12
Lung	4.10 ± 0.46	3.23 ± 0.03	3.93 ± 0.46	3.25 ± 0.49
Kidney	4.20 ± 0.25	4.36 ± 1.14	3.87 ± 0.31	3.08 ± 0.42
Liver	2.29 ± 0.19	1.79 ± 0.17	2.74 ± 0.10	11.29 ± 0.63
Spleen	3.41 ± 0.32	3.16 ± 0.38	2.93 ± 2.93	9.05 ± 1.01
Ovary	4.33 ± 0.43	3.49 ± 0.16	2.94 ± 0.43	1.47 ± 0.03
Muscle	0.97 ± 0.06	0.90 ± 0.14	0.89 ± 0.19	0.54 ± 0.03
Brain	0.20 ± 0.01	0.13 ± 0.01	0.19 ± 0.01	0.14 ± 0.01