PLGA Nanoparticles for Ultrasound-Mediated Gene Delivery to Solid Tumors
PEG-PLGA particles as ultrasound contrast agents in vivo. (a) Ultrasound images of mouse pancreatic tumors obtained in a nonlinear imaging mode before injection (1)–(3) and after intratumoral injection of plain nanocapsules (2) or PEGylated nanocapsules (4). The tumor is indicated as the region of interest (ROI) represented by a circle. (b) Confocal microscopy images of tumor slices from a control mouse (control) and mice after 24 h of an intravenous injection of non-PEGylated nanocapsules (NC) and PEGylated nanocapsules (NCL-PEG). DIC corresponds to differential interference Nomarski contrast. Red fluorescence corresponds to PLGA dyed with Nil Red. Reprinted from  with permission from Elsevier.