Development of Oral Sustained Release Rifampicin Loaded Chitosan Nanoparticles by Design of Experiment

Table 1

2³ full-factorial design of independent and dependent parameters (𝑛=3).

(a)

Batch code	Independent variables			Dependent factors (response value)			Overall desirability (OD)
	𝑋1^a	𝑋2^b	𝑋3^c	Average particle size (nm) (𝑌1)	% drug entrapment efficiency (𝑌2)	% drug loading (𝑌3)
CN1	−1	−1	−1	199.5	20.17±6.53	23.05±8.19	0.3323
CN2	+1	−1	−1	243.0	42.89±1.93	43.96±2.33	0.8148
CN3	−1	+1	−1	180.5	22.07±1.98	23.56±2.74	0.3860
CN4	+1	+1	−1	221.9	44.17±1.98	42.96±2.91	0.8558
CN5	−1	−1	+1	264.2	14.40±5.48	15.90±5.82	0.0002
CN6	+1	−1	+1	383.3	24.27±2.73	23.78±1.75	0.0000
CN7	−1	+1	+1	226.3	23.03±4.07	26.12±4.14	0.4061
CN8	+1	+1	+1	278.2	49.36±5.19	45.17±5.15	0.8034

(b)

Variables	Levels
	Low (−1)	High (+1)
𝑋1^a	1	2
𝑋2^b	19,000	26,000
𝑋3^c	0.1	0.2

aConcentration of Chitosan (%w/v), bspeed of homogenization (rpm), and cconcentration of TPP (%w/v).