Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase

Table 1

Fractional factorial design showing the effect of independent variables on the outcome.

Run order	PC (A) (%w/v)	Ch (B) (%w/v)	(C) °C	SPR (D) rpm	% PE
1	50	0.5	40	200	10.22
2	200	0.125	40	200	7.69
3	200	0.5	40	50	5.51
4	50	0.125	60	200	9.7
5	200	0.125	60	50	8.38
6	50	0.5	60	50	10.38
7	200	0.5	60	200	6.17
8	50	0.125	40	50	10.58
Effect	−3.28	−1.02	0.16	−0.27	NA
	AB—1.18	AC—0.52	AD—0.25	NA
% cont.	79.12	7.59	0.18	0.52
	AB—10.18	AC—1.96	AD—0.46	NA
value
OWA	0.003	0.51	0.921	0.87
TWA	AB—0.022	AC—0.55	AD—0.78	NA

PC: phosphatidylcholine; Ch: cholesterol; : temperature during film formation (solvent evaporation); SPR: speed of rotation (during film formation); % PE: percentage of protein encapsulation; % cont.: percentage contribution.
OWA: one-way ANOVA; TWA: two-way ANOVA; AB, AC, and AD: interaction between phosphatidylcholine-cholesterol, phosphatidylcholine-temperature, and phosphatidylcholine-speed of rotation, respectively. NA: not applicable.