Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase

Table 2

Central composite design showing the effect of independent variables on the dependent variable.

Std. order	Run order	PC (A) (%w/v) (A)	SPR (rpm) (B)	% PE	Size (nm)	ZP (mV)
3	1	40	225	9.58	562.9	−49.2
5	2	22.40	137.5	10.02	1388	−42.9
7	3	82.5	13.76	9.00	837.8	−60.1
8	4	82.5	261.24	9.42	505.2	−54
6	5	142.60	137.5	8.60	273.8	−59.6
9	6	82.5	137.5	8.67	557.2	−64.3
10	7	82.5	137.5	8.93	417.8	−51.3
2	8	125	50	8.67	558.3	−58.6
12	9	82.5	137.5	8.49	1110	−65
1	10	40	50	9.93	1978	−56.4
4	11	125	225	8.27	737	−64.8
11	12	82.5	137.5	8.11	446.5	−63.3
13	13	82.5	137.5	8.96	514.3	−51.3
		PC (A)		0.005	0.006	0.032
value		SPR (B)		0.902	0.06	0.57
		A * B		0.961	0.02	0.276

PC: phosphatidyl choline; SPR: speed of rotation (during film formation); % PE: percentage of protein encapsulation; ZP: zeta potential.