Research Article
Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase
Table 2
Central composite design showing the effect of independent variables on the dependent variable.
| Std. order | Run order | PC (A) (%w/v) (A) | SPR (rpm) (B) | % PE | Size (nm) | ZP (mV) |
| 3 | 1 | 40 | 225 | 9.58 | 562.9 | −49.2 | 5 | 2 | 22.40 | 137.5 | 10.02 | 1388 | −42.9 | 7 | 3 | 82.5 | 13.76 | 9.00 | 837.8 | −60.1 | 8 | 4 | 82.5 | 261.24 | 9.42 | 505.2 | −54 | 6 | 5 | 142.60 | 137.5 | 8.60 | 273.8 | −59.6 | 9 | 6 | 82.5 | 137.5 | 8.67 | 557.2 | −64.3 | 10 | 7 | 82.5 | 137.5 | 8.93 | 417.8 | −51.3 | 2 | 8 | 125 | 50 | 8.67 | 558.3 | −58.6 | 12 | 9 | 82.5 | 137.5 | 8.49 | 1110 | −65 | 1 | 10 | 40 | 50 | 9.93 | 1978 | −56.4 | 4 | 11 | 125 | 225 | 8.27 | 737 | −64.8 | 11 | 12 | 82.5 | 137.5 | 8.11 | 446.5 | −63.3 | 13 | 13 | 82.5 | 137.5 | 8.96 | 514.3 | −51.3 |
| | PC (A) | 0.005 | 0.006 | 0.032 | value | SPR (B) | 0.902 | 0.06 | 0.57 | | A * B | 0.961 | 0.02 | 0.276 |
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PC: phosphatidyl choline; SPR: speed of rotation (during film formation); % PE: percentage of protein encapsulation; ZP: zeta potential.
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