Sustained Release of Protein Therapeutics from Subcutaneous Thermosensitive Biocompatible and Biodegradable Pentablock Copolymers (PTSgels)

Figure 7

In vivo imaging of mice after subcutaneous injection of PTSgel. Mice were injected with PBS, PTSgel (10% and 20% 10GH or 113GH), or a combination of NIR-IgG PBS or NIR-IgG PTSgel in triplicate. Mice were anesthetized and imaged prior to injection, immediately after injection, and then on days 1–5, 7, 10, and 14 after injection and then weekly thereafter using an in vivo imager (IVIS, Xenogen, Alameda, CA) ((a) and (c)). Quantification of fluorescence was measured using the imaging software automatic region of interest (ROI) setting to calculate the radiant efficiency of the injection site ((b) and (d)).

(a)

(b)

(c)

(d)