Statistical Optimization of Fermentation Process Parameters by Taguchi Orthogonal Array Design for Improved Bioethanol Production

Table 7

Validation of Taguchi experimental data values.

Factor/parameter	Taguchi optimum	Experiment optimum
Recombinant GH5 cellulase; (5.7 U mg^−1, 0.45 mg mL^−1) (%, v v^−1)	1.0	1.0
Recombinant GH43 hemicellulase; (3.7 U mg^−1, 0.32 mg mL^−1) (%, v v^−1)	2.0	0.5
S. cerevisiae (3.6×10^8 cells mL^−1); (%, v v^−1)	1.5	1.5
C. shehatae (2.1×10^8 cells mL^−1); (%, v v^−1)	0.25	0.25
pH	4.3	5.0
Temperature (°C)	35	35
𝑆/𝑁 ratio	−10.95	−11.99
Response experimental; ethanol titre (%, v v^−1)	0.2540	0.2512
Response predicted (%, v v^−1); ethanol titre (%, v v^−1)	0.2705	0.2677
Ethanol titre (g L^−1)	2.00	1.98
Ethanol yield; (g of ethanol g of substrate^−1)	0.200	0.198