Research Article
Histochemical and Immunohistochemical Study of Peripolar Cells in Sheep
Table 1
Primary antibodies used and informative data.
| Antibody | Supplier | Dilution | Antigen retrieval | Primary antibody—incubation time |
| α-Smooth muscle actin | Novocastra (αsm-1) | 1 : 50 | Microwave—5 minutes | 3 h RT | NSE | Zymed (Lote 20771997) | 1 : 50 | Microwave—5 minutes | 2 h RT | Chromogranin A | Dako | 1 : 50 | Microwave—5 minutes (2x) | 2 h RT | S100 protein | Novocastra | 1 : 200 | Microwave—5 minutes | 1 h RT | Desmin | Dako (D33) | 1 : 100 | Microwave—5 minutes | 2 h RT | Cox-2 | Transduction Laboratories, Kentucky (Clone SP-21) | 1 : 50 | Microwave—5 minutes (4x) | ON at 4.°C | AE1/AE3 | Dako (AE1/AE3) | 1 : 50 | Microwave—5 minutes | 2 h RT | Wide Spectrum Cytokeratin | Dako | 1 : 1000 | Microwave—5 minutes (2x) | 2 h RT |
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RT: room temperature; ON: overnight.
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