Table of Contents
Journal of Histology
Volume 2013, Article ID 981305, 5 pages
Research Article

A Study of Changes in Morphology of Osteoarthritic Articular Cartilage Using Computerized Image Analysis

1Department of Anatomy, Christian Medical College, Ludhiana, India
2Department of Anatomy, Swami Devi Dayal Hospital and Dental College, Golpura, Barwala, Haryana, India

Received 5 February 2013; Revised 1 April 2013; Accepted 2 April 2013

Academic Editor: Luigi F. Rodella

Copyright © 2013 Neeru Goyal and Madhur Gupta. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Histological studies on articular cartilage have been traditionally based on individual observations but this approach is limited by its subjectivity and bias, yielding considerable variability. So the present study was conducted to observe the various changes in the morphology of osteoarthritic femoral articular cartilage using computerized image analysis. The cartilage specimens were divided into two groups: group 1 ( ) (46–81 years) consisted of OA specimens. Group 2 ( ) (41–86 years) consisted of non-OA specimens. A 5 μm thick paraffin sections were stained with H&E staining and analyzed using Image-Pro Express image analysis software for quantitative analysis of articular cartilage. Various parameters, namely, total thickness of the cartilage, area of lacunae in each zone, area of subchondral cavities, and number of chondrocytes per 10,000 μm2 area in each zone, were measured. Microscopic appearance of OA cartilage was much different as compared to control. Various changes seen were different in all specimens and they were not related to age. Lacunar size in all four zones was found to differ significantly in the OA (group 1) and control (group 2) ( ). The results suggest that OA should be considered as a specific process and not simply as an inevitable feature of ageing.