The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida
Figure 2
Photomicrographs of glycoconjugates in the epidermal mucous cells of Eisenia foetida. (a) PAS staining: neutral glycoconjugates in epidermal mucous cells of second region (arrows). (b) KOH/PAS staining: glycoconjugates with sialic acid residues in epidermal mucous cells of first region (arrows). (c) AF staining: glycoconjugates with sulfate in epidermal mucous cells of second region (arrows). (d) PAS/AB pH 2.5 staining: neutral and acidic glycoconjugates, first region. Thin arrow: PAS (+); thick arrow: AB pH 2.5 (+). (e) AB pH 2.5 staining: acidic glycoconjugates with carboxylated and sulfated esters in epidermal mucous cells of second region (arrows). (f) AB pH 0.5 staining: very sulfated glycoconjugates in epidermal mucous cells of first region (arrows). Scale bar: 50 μ.
