Research Article

The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida

Table 1

Conventional histochemical staining procedures for visualizing and identifying glycoconjugates in epidermal mucous cells of Eisenia foetida.

ProceduresGlycoconjugates unveiledReferences

PASNeutral Glycoconjugates[35]
PAS/AB pH 2.5To distinguish between neutral and acidic glycoconjugates[36]
AB pH 0.5Very sulfated glycoconjugates[37]
AB pH 1.0Glycoconjugates with O-sulfate esters[37]
AB pH 2.5Acidic glycoconjugates with carboxylated and
sulfated esters
[37]
AFGlycoconjugates with sulfate[38]
AF/AB pH 2.5To distinguish between sulfated glycoconjugates and acidic glycoconjugates[39]
KOH/PASGlycoconjugates with sialic acid residues [40]

AB: alcian blue; PAS: periodic acid Schiff reagent; AF: aldehyde fuchsin; KOH: potassium hydroxide.