Research Article
The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida
Table 1
Conventional histochemical staining procedures for visualizing and identifying glycoconjugates in epidermal mucous cells of Eisenia foetida.
| Procedures | Glycoconjugates unveiled | References |
| PAS | Neutral Glycoconjugates | [35] | PAS/AB pH 2.5 | To distinguish between neutral and acidic glycoconjugates | [36] | AB pH 0.5 | Very sulfated glycoconjugates | [37] | AB pH 1.0 | Glycoconjugates with O-sulfate esters | [37] | AB pH 2.5 | Acidic glycoconjugates with carboxylated and sulfated esters | [37] | AF | Glycoconjugates with sulfate | [38] | AF/AB pH 2.5 | To distinguish between sulfated glycoconjugates and acidic glycoconjugates | [39] | KOH/PAS | Glycoconjugates with sialic acid residues | [40] |
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AB: alcian blue; PAS: periodic acid Schiff reagent; AF: aldehyde fuchsin; KOH: potassium hydroxide.
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