PRKRA Localizes to Nuage Structures and the Ectoplasmic Specialization and Tubulobulbar Complexes in Rat and Mouse Testis

Figure 3

Localization of PRKRA in nuage structures by IEM. Gold particles indicate PRKRA antigenic sites. (a, c, e, and g) Samples from rat. (b, d, f, and h) Samples from mouse. All insets show IF images corresponding to IEMimages (arrowheads). (a, b) Aggregates of 60–90 nm particles (60–90P) were labeled with gold particles (circles). (a) A rat pachytene spermatocyte at stage X. (b) A mouse pachytene spermatocyte at stage IX. (c, d) PRKRA stainedirregularly-shaped perinuclear granules (ISPG). (c) A rat pachytene spermatocyte at stage IX. (d) A mouse pachytene spermatocyte at stage IX. (e, f) PRKRA localized to intermitochondrial cement (IMC) between mitochondria (M) (arrows). (e) A rat spermatocyte at stage X. (f) A mouse spermatocyte at stage IX. (g, h) Chromatoid bodies (CB) were stained for PRKRA. (g) A rat step 5 spermatids. (h) A mouse step 4 spermatid. (i) Control for 60–90P. (j) Control for ISPG. (k) Control for IMC. (l) Control for CB. Bar = 25 μm for insets, 0.5 μm for all IEM images.