Journal of Histology / 2014 / Article / Fig 4

Research Article

PRKRA Localizes to Nuage Structures and the Ectoplasmic Specialization and Tubulobulbar Complexes in Rat and Mouse Testis

Figure 4

PRKRA staining in regions surrounding the sickle-shaped heads of late spermatids of rat. (a) An IF image. Strong punctate staining was observed at both the ventral (arrows) and dorsal (arrowheads) sides of sickle-shaped heads. (b) IEM staining of PRKRA. Gold labeling associated with dense material in the ectoplasmic specialization of Sertoli cell (arrows) and also with dense material attached to the tubulobulbar complexes in the Sertoli cell cytoplasm (SC) (arrowheads). A vacuole in the Sertoli cell was negative for PRKRA ( ). (c) Control. Note that no gold labeling was observed in the dense material at the ectoplasmic specialization (arrows), that attached to the tubulobulbar complexes, and the vacuole ( ). N, nucleus. Bar = 10 μm for (a) and = 1 μm for (b) and (c).