Table of Contents
Journal of Histology
Volume 2016, Article ID 7680701, 8 pages
Research Article

Histochemical and Molecular Characterization of Spongiosal Cells in Native Tissue, Two- and Three-Dimensional Cultures of Rat Aortic Valve

1Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology, Hyderabad 500007, India
2Birla Institute of Technology and Sciences, K. K. Birla Campus, Zuarinagar, Goa 403726, India

Received 7 September 2015; Revised 2 December 2015; Accepted 15 December 2015

Academic Editor: Victor H. Casco

Copyright © 2016 Vibudha Nanduri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: (A) Histological studies of the 3D reconstructed AV tissue grown in CM, using collagen gel as a scaffold, at 20X magnification. (B) Immunofluorescence of the AV tissue grown in 3D cultures for 30 days in CM, at P0 and 40X magnification. DAPI (blue stain) stained nuclei; Alexafluor 594 nm (red stain) fluorescence for different markers as indicated. Scale bar is 100 μm.

  1. Supplementary Material