The Effect of 17β-Estradiol Administration on Cutaneous Wound Healing in 24-Week Ovariectomized Female Mice
Figure 4
Re-epithelialization, collagen deposition, and wound contraction. (a) Proportion of no re-epithelialization. There were no significant differences between the three groups on days 3–14. (b) Hematoxylin and eosin sections on day 7. Bar 500 μm. (c) The ratio of collagen fibers. There were no significant differences between the three groups, although that in the OVX + 17β-estradiol group tended to be increased compared with that in the OVX group on days 7 and 11. (d) Azan-stained sections on day 7. Bar 500 μm. (e) The ratio of myofibroblasts. The ratio of myofibroblasts in the OVX + 17β-estradiol group increased significantly compared with that in the OVX group on day 11. However, there were no significant differences between OVX + 17β-estradiol and SHAM groups or SHAM and OVX groups. (f) Myofibroblasts stained with anti--SMA antibody were observed in granulation tissue on day 11. Bars 500 μm (SHAM), 1000 μm (OVX), and 200 μm (OVX + 17β-estradiol). Note a bridge-like structure (arrows) formed by the myofibroblasts. Values are expressed as mean ± SD, for each group, ANOVA, Tukey-Kramer; : OVX versus OVX + 17β-estradiol.
