Table of Contents
Journal of Medical Engineering
Volume 2013, Article ID 560328, 12 pages
Research Article

Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis

1Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, USA
2Biomedical Engineering Program, Louisiana Tech University, Ruston, LA 71272, USA
3School of Bio Sciences & Technology, VIT University, Vellore 632014, India
4Biomedical Technology Department, King Saud University, Riyadh 11433, Saudi Arabia
5Biomedical Engineering Program, Texas A&M University, College Station, TX 77843, USA
6School of Biological Sciences, Louisiana Tech University, Ruston, LA 71272, USA

Received 16 February 2013; Revised 24 April 2013; Accepted 25 April 2013

Academic Editor: Rad Zdero

Copyright © 2013 Jameel Shaik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cell patterning has emerged as an elegant tool in developing cellular arrays, bioreactors, biosensors, and lab-on-chip devices and for use in engineering neotissue for repair or regeneration. In this study, micropatterned surfaces were created using the layer-by-layer lift-off (LbL-LO) method for analyzing canine chondrocytes response to patterned substrates. Five materials were chosen based on our previous studies. These included: poly(dimethyldiallylammonium chloride) (PDDA), poly(ethyleneimine) (PEI), poly(styrene sulfonate) (PSS), collagen, and chondroitin sulfate (CS). The substrates were patterned with these five different materials, in five and ten bilayers, resulting in the following multilayer nanofilm architectures: (PSS/PDDA)5, (PSS/PDDA)10; (CS/PEI)4/CS, (CS/PEI)9/CS; (PSS/PEI)5, (PSS/PEI)10; (PSS/Collagen)5, (PSS/Collagen)10; (PSS/PEI)4/PSS, (PSS/PEI)9/PSS. Cell characterization studies were used to assess the viability, longevity, and cellular response to the configured patterned multilayer architectures. The cumulative cell characterization data suggests that cell viability, longevity, and functionality were enhanced on micropatterned PEI, PSS, collagen, and CS multilayer nanofilms suggesting their possible use in biomedical applications.