Table of Contents
Journal of Mycology
Volume 2013 (2013), Article ID 358140, 7 pages
http://dx.doi.org/10.1155/2013/358140
Research Article

Screening of Fusarium graminearum Isolates for Enzymes Extracellular and Deoxynivalenol Production

Research and Development Center for Industrial Fermentations (CINDEFI), UNLP, CCT-La Plata, CONICET, School of Science, La Plata National University, B1900ASH La Plata, Argentina

Received 30 May 2013; Revised 20 September 2013; Accepted 10 October 2013

Academic Editor: Maria João Sousa

Copyright © 2013 Leonel M. Ortega et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fusarium graminearum, the main etiological agent of Fusarium head bligh, has high intraspecific genetic diversity, which is related to the variability in the aggressiveness among isolates against wheat. The aggressiveness involves different mechanisms as the production and liberation of extracellular enzymes and mycotoxins. In the present paper, several F. graminearum isolates obtained from wheat spikes from Pampas region, Argentina, were screened for polygalacturonase (pectinase), proteolytic, and lipase extracellular enzymatic activities production, as well as for the capacity to produce deoxynivalenol. The enzymatic production in terms of magnitude was varied among isolates, which could be related to a differential capacity to infect wheat. Both polygalacturonase as proteolytic activities had a maximum activity in the first days of incubation. Instead, the lipase activity reached its maximum activity after advanced incubation time. Deoxynivalenol production was delayed over time with respect to the first enzymatic activities, which would infer its relation to the progress of the disease in the host, more than with the early stages of infection. The characterization carried out in this research would allow us to apply a selection criterion among isolates for further research.