Tetrazolium/Formazan Test as an Efficient Method to Determine Fungal Chitosan Antimicrobial Activity

Table 1

The sensitivity of TTC method to determine the survival rate of treated bacterial strains with chitosan, compared to plate count method.

Chitosan conc. (%)	S. typhimurium ∗		Average correlation %	S. aureus ∗		Average correlation %
	Relative colony count (%)	Relative formazan absorbance %		Relative colony count (%)	Relative formazan absorbance %
Control (0)	100	100		100	100
0.1	59.93	58.38	97.41	61.05	59.34	97.20
0.2	26.85	27.22	101.38	29.93	28.78	96.16
0.3	13.81	14.13	102.32	16.94	17.17	101.36
0.4	1.92	1.89	98.44	6.41	6.56	102.34
0.5	0.42	0.40	95.24	2.81	2.91	103.56
	General correlation mean		98.96	General correlation mean		100.12

∗Treated bacteria were incubated for 3 h at 37°C before testing.