Table of Contents
Journal of Mycology
Volume 2014, Article ID 371218, 5 pages
Research Article

Antagonistic Evaluation of Bioactive Metabolite from Endophytic Fungus, Aspergillus flavipes KF671231

1Department of Biotechnology, Barkatullah University, Bhopal 462026, India
2Department of Microbiology, Barkatullah University, Bhopal 462026, India

Received 25 May 2014; Revised 14 July 2014; Accepted 15 August 2014; Published 27 August 2014

Academic Editor: Zia U. Khan

Copyright © 2014 Ankita Verma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Of the total 40 endophytic fungi isolated from foliar tissues of medicinal plant Stevia rebaudiana Bertoni, a fungal isolate, Aspergillus flavipes, was subjected to bioassay guided fractionation. The fractionation was found active against medicinal plant pathogen Sclerotinia sclerotiorum with an inhibition zone of 29 mm in size. Further the metabolite was extracted which shows 20% growth inhibition in 24 h and 46% after 48 h, respectively. Bioassay guided chemical compound was identified as 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester. On the basis of morphological characters and rDNA sequencing of ITS region the endophyte was identified as Aspergillus flavipes which showed promising plant growth promotory properties.