Table of Contents
Journal of Mycology
Volume 2014, Article ID 582672, 14 pages
http://dx.doi.org/10.1155/2014/582672
Research Article

Genetic Diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain

1Department of Agroforestry Sciences, Escuela Técnica Superior de Ingenieríaa Agronómica (ETSIA), University of Seville, Carretera de Utrera Km 1, 41013 Seville, Spain
2Department of Plant Protection, University of Sassari, Via Enrico de Nicola 9, 07100 Sassari, Italy

Received 18 February 2014; Accepted 20 May 2014; Published 6 July 2014

Academic Editor: Praveen Rao Juvvadi

Copyright © 2014 Raúl Castaño et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The diversity of races and prevalence of pathogenic populations of Fusarium oxysporum f. sp. dianthi (Fod) were surveyed in an area in southern Spain. From 54 farms, 132 isolates were collected from wilted carnation plants. Isolates were characterized by RAPD-PCR, DNA sequence analysis of the TEF1-α gene, and race-specific molecular markers. Selected isolates from RAPD groups were phenotypically evaluated by pathogenicity tests. Data analysis showed that Fod race 2 was the most frequent and prevalent race in the study area, followed by race 1/8. Moreover, phylogenetic analyses showed similar results, which were different to those of the race-specific PCR assays. It was concluded that (i) seven isolates were not classified in groups where Fod testers were clustered; even they showed different results when race-specific markers were used, (ii) ten isolates with retarded race 1 or race 8 specific band were characterized as F. proliferatum by TEF1-α gene sequencing and clustered into an outgroup, and (iii) six isolates failed to generate an amplification signal using race-specific markers. Furthermore, three of them were grouped close to race 2 tester according to the phylogenetic analyses, showing the same differential pathogenicity as race 2. This may indicate a Fod race 2 subgroup in this region.