Genetic Diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain

Figure 4

Specific Fod-race primers showing different amplification products. Agarose gel electrophoresis (1.5%) of PCR products from the genomic DNA of 12 representative RAPD group isolates, four Fod testers, and one Fusarium redolens isolate. (a) Multiplex PCR in which five primers were mixed in the same reaction tube, where combinations of Ft3/R8.1, Ft3/R2.1, and Ft3/R4.2 resulted in bands of 295 (race 1/8), 564 (race 2), and 1315 bp (race 4), respectively; bands with retarded electrophoretic mobility are shown with an arrow. (b) Multiplex PCR products obtained with the LSU (not shown) and Fusarium redolens-specific primers, with a resulting band of 386 bp. (c) PCR of the eubacterial primers 704f and 1495r where a 900 bp product was not amplified. Fod testers R1 (race 1), R2 (race 2), R4 (race 4), and R8 (race 8); Fr: Fusarium redolens and 911 (bacterial tester). M: molecular size marker (1 kb ladder, Biotools); sizes (base pairs) are indicated on the left.