Table of Contents
Journal of Mycology
Volume 2016 (2016), Article ID 8502629, 13 pages
http://dx.doi.org/10.1155/2016/8502629
Research Article

Phylogenetic Analysis of Nucleotide Sequences from the ITS Region and Biological Characterization of Nematophagous Fungi from Morelos, Mexico

1Helminthology Department, National Centre for Disciplinary Research in Veterinary Parasitology, CENID-Parasitología Veterinaria, INIFAP, Boulevard Paseo Cuauhnáhuac No. 8534, Colonia Progreso, 62550 Jiutepec, MOR, Mexico
2Department of Research, Polytechnic University of Morelos State (UPEMOR), Boulevard Paseo Cuauhnáhuac No. 566, Colonia Lomas del Texcal, 62550 Jiutepec, MOR, Mexico

Received 29 December 2015; Revised 13 March 2016; Accepted 3 April 2016

Academic Editor: Dagmar Triebel

Copyright © 2016 Gloria Sarahí Castañeda-Ramírez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We determined the morphological taxonomy of eighteen nematophagous fungi (NF), as well as their in vitro predatory activity against Haemonchus contortus infective larvae (L3). Fungi were classified into six genera and three species, the most common of which were Monacrosporium eudermatum and Arthrobotrys oligospora. We then sequenced five NF isolates using ITS4 and ITS5 primers. These sequences showed high identity with sequences from the NCBI database (98-99%). In contrast, alignments among the same genera and species demonstrated 83–97% identity. Polymorphisms observed between Arthrobotrys and Monacrosporium appear to be associated with differences in biological function, nonspecific mutations, evolutionary processes, feeding behaviour, predatory activity, and microecosystems.