Replication Past the 𝛾-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase 𝜂

Figure 2

Extension of a 14-mer primer by varying concentration (5, 10, 15, 20, and 50 nM) of hPol 𝜂 (a) and yPol 𝜂 (b) on control and G[8,5-Me]T templates in the presence of all four dNTPs. The experiments were carried out at 37^∘C for 30 min.

(a)

(b)