Replication Past the -Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase
Figure 4
Single nucleotide incorporation and extension assay. Template-primer (50 nM) was incubated with 6.4 nM hPol or yPol for various times with increasing concentrations of dNTP. Steady-state kinetics for single nucleotide incorporation opposite cross-linked (G) (solid line) or control (G) (dashed line) are shown in (a), (b), (e), and (f). (a) and (b) represent dCTP and dATP incorporations, respectively, for hPol whereas (e) and (f) represent the same for yPol . Steady-state kinetics for dGTP incorporation opposite (C) immediately following the cross-linked (G) (solid line) or control (G) (dashed line) are shown in (c), (d), (g), and (h). (c) and (d) represent extension of G*:C and G*:A pairs, respectively, for hPol whereas (g) and (h) represent the same for yPol . Error bars show the standard deviation of at least three experiments.
