Replication Past the -Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase
Figure 6
(a) Types and frequencies of mutations induced by G[8,5-Me]T as determined from the full-length extension products generated by hPol (top) and yPol (bottom). It is noteworthy that no mutants were isolated from the control batches after sequencing in excess of one hundred colonies. (b) The combined data in (a) is represented in bar graph showing the percentages of each type of single-base substitution or deletion induced by G[8,5-Me]T by hPol (top) and yPol (bottom). The colors represent T (green), A (blue), G (red), C (orange), and one-base deletion (yellow). The T deletion in a run of three thymines by hPol was arbitrarily shown here at the T closest to the lesion.
