Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil

Table 1

Crystallization conditions.

Crystals	fUA^1	fUA^2	fUA^3	fUA^4	fUG^1	fUG^2	fUG^3
Droplet
Sodium cacodylate buffer solution (mM)	20	20	20	20	20^a	20	20
pH	6.5	6.0	7.0	6.0	8.1	7.0	7.0
DNA (mM)	0.5	0.5	0.5	0.5	0.5	0.5	0.5
Spermine 4HCl (mM)	6	6	6	6	6	6	6
Sodium chloride (mM)	6	40	40	6	—	40	40
Potassium chloride(mM)	60	—	—	40	—	—	—
Magnesium chloride(mM)	6	10	—	—	—	10	10
Barium chloride(mM)	—	—	10	—	—	—	—
MPD (% in v/v)	5	5	5	5	5	5	5
Hoechst 33258 (mM)	—	—	—	—	—	0.5	—
DAPI (mM)	—	—	—	—	—	—	0.5
Reservoir solution
MPD (%)	40	40	40	40	40	35	40
Temperature (K)	277	277	277	277	277	277	277

MPD: 2-methyl-2,4- pentanediol.
Hoechst 33258: 2^-(4-hydroxyphenyl)-6-(4- methyl-1-piperazinyl)-2,6^-bi-1H-benzimidazole.
DAPI: 4^,6-diamidino-2-phenylindole.
^aBuffer solution of fUG¹ is 3-[4-(2-Hydroxyethyl)-1-piperazunyl]propanesulfonic acid.