Journal of Nucleic Acids

Research Article

Cation Involvement in Telomestatin Binding to G-Quadruplex DNA

Figure 2

Negative ion mode electrospray mass spectra of mixtures of 10  𝜇 M telomestatin and 5  𝜇 M of G-quadruplexes. Zooms on the peaks of free G-quadruplex (a) [ T G 4 T ] 4 , (b) telomeric sequence ( T 2 A G 3 ) 4 , and (c) Pu22myc promoter sequence G A G 3 T G 4 A G 3 T G 4 A 2 G , and on the complexes between (d) one telomestatin and [ T G 4 T ] 4 , (e) one telomestatin and ( T 2 A G 3 ) 4 , and (f) one telomestatin and G A G 3 T G 4 A G 3 T G 4 A 2 G . The spectra were recorded from and 80/20 aqueous ammonium acetate (150 mM)/methanol solution, using soft source conditions to preserve the specifically bound ammonium ions.
121259.fig.002