Journal of Nucleic Acids

Research Article

Design, Synthesis, and Analysis of Minor Groove Binder Pyrrolepolyamide- -Deoxyguanosine Hybrids

Figure 2

CD spectra for the DNA duplexs with ligand (Hybrid 2, Hybrid 3, Dst, or compound 11) at several [Iigand]/[duplex] ratios in buffer consisting of 10 mM sodium phosphate, 10 mM NaCL, and 0.1 mM Na2EDTA at pH 7.0 and 20°C: 3.0 mL of duplex solution (5.8  𝜇 M) titrated ligand (Hybrid 2, Hybrid 3, and Dst: 0–14.5  𝜇 M) and 3.0 mL of duplex solution (4  𝜇 M) titrated ligand (compound 11: 0–12  𝜇 M). *DNA duplexs: DNA 1  = d-( 5 -CGCAAATTGGC- 3 )-d-( 3 -GCGTTTAACCG- 5 ), DNA 2  = d-( 5 -CGCAGACTGGC- 3 )-d-( 3 -GCGTCTGACCG- 5 ), DNA 3  = d-( 5 -GCACGACTACG- 3 )-d-( 3 -CGTGCTGATGC- 5 ), DNA 1 contains the high affinity binding site ( 5 -AAATT- 3 ) of Dst.
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