Targeting the OB-Folds of Replication Protein A with Small Molecules
Figure 6
EcSSB protein-ssDNA interaction is not affected by the RPA inhibitors. (a) Titration of TDRL-505 in EcSSB DNA-binding reactions. TDRL-505 (0, 10, 20, 30, 40, 50, 75, and 100 μM) was preincubated with EcSSB, and binding to a 30-base pair ssDNA was assessed by EMSA as described in “Section 2.” (b) Analysis of TDRL-505 inhibition of EcSSB. The average of three independent experiments is presented with SD. (c) CheSS19 titration in EcSSB DNA-binding reactions. CheSS19 (0, 25, 50, 100, and 200 μM) was preincubated with EcSSB, and binding reactions were carried out as in (a). (d) Analysis of CheSS19 inhibition of EcSSB. The average of three independent experiments is presented with SD. (e) Titration of MCI13E and MCI13F in EcSSB DNA-binding reactions. Concentrations of MCI13E/F used were 0, 25, 50, 100, and 200 μM. (f) Analysis of MCI13E/F inhibition of EcSSB. The average of three independent experiments is presented with SD. Data for MCI13E is shown as filled circles, and MCI13F is shown as open circles. In panels (a), (c), and (e), the “Free” ssDNA is indicated with an arrow, and the “Bound” ssDNA is indicated by brackets. All reactions were performed in the same order and manner as the RPA-binding reactions.
