Effects of different concentrations of estrogen and variant haplotypes of CYP1B1 and APE1 on the formation and repair of DNA adducts. (a) Shows the effect of doubling the concentration of on the number of AP lesions per 105 nucleotides over time using (red curve) and (blue curve). (b) Shows the effect of variant haplotypes of CYP1B1 and APE1 leading to an increase in the number of AP lesions per 105 nucleotides relative to wild-type enzymes. Each curve uses different assumptions about the kinetic constant, , for the CYP1B1 and APE1 enzymes. The red curve depicts the wild-types with (CYP1B1) and (APE1). The blue curve uses a 30% increase in , keeping . The green curve employs a 30% reduction in , keeping . Finally, the purple curve uses both a 30% increase in and a 30% reduction in . (c) Shows the effect of different variant haplotypes of CYP1B1 and APE1 that cause a decrease in the number of AP lesions per 105 nucleotides relative to wild-type enzymes. The wild-type is shown in red, a 30% decrease of CYP1B1 activity with APE1 activity held at its wild-type level in blue, 30% increase of APE1 activity with CYP1B1 activity held at its wild-type level in green, and a 30% decrease in CYP1B1 activity with a 30% increase of APE1 activity in purple.
