Repair of DNA Alkylation Damage by the Escherichia coli Adaptive Response Protein AlkB as Studied by ESI-TOF Mass Spectrometry

Table 1

Calculated and observed monoisotopic molecular weight of oligonucleotides and intermediates present in the AlkB repair reactions.

Lesion or base	MW (calculated)	m/z (calculated)	m/z (observed)
	of neutral species	−4 charge monoisotopic peak	−4 charge monoisotopic peak
G	4904.86	1225.21	1225.11
1MeG	4918.87	1228.71	1228.61/2
𝑂^6MeG	4918.87	1228.71	1228.62/3
A	4888.86	1221.21	1221.12/23
1MeA	4902.88	1224.71	1224.67
eA	4912.86	1227.21	1227.22/4
eA epoxide	4928.86	1231.21	1231.23
eA glycol	4946.87	1235.71	1235.72
C	4864.85	1215.20	1215.20/1
3MeC	4878.87	1218.71	1218.72
3EtC	4892.88	1222.21	1222.23
5MeC	4878.87	1218.71	1218.73
eC	4888.85	1221.20	1221.21/2
eC epoxide	4904.84	1225.20	1225.19
eC glycol	4922.86	1229.71	1229.69
T	4879.85	1218.95	1218.97
3MeT	4893.87	1222.46	1222.49