Mus308 Processes Oxygen and Nitrogen Ethylation DNA Damage in Germ Cells of Drosophila

Table 1

Recessive lethal (RL) and v mutation frequencies induced by DES on postmeiotic male germ cells of D. melanogaster, under mus308 deficient (𝑚𝑢𝑠308⁻) and efficient (𝑚𝑢𝑠308⁺) conditions. Values of mutability index (MI) and their statistical signification are also presented.

			F1 Analysis			F2 Analysis
Repair status	Treat.	Dose (mM)	Offspring	𝜈 mutants	Freq.  (×10^−4)	Offspring(a)	𝜈 mutants	Freq. (×10^−4)	%RL	MI^(b) (Mmus 308^−/Mmus 308^+)
mus308 −	Control		24002	0	0	15698	0	0	0.17
	DES	10	19582	0	0	12576	0	0	3.04∗∗∗	1.55∗∗∗
		15	154737	2	0.13	56093	11	1.96	2.91∗∗∗	0.13∗∗∗
		25	10092	0	0	6925	0	0	0.51∗∗∗	0.01∗∗∗
mus308 +	Control		8678	0	0	6766	0	0	0.28
	DES(c)	10	54175	0	0	13090	2	1.53	2.13∗∗∗
		15	23209	4	1.72	9101	13	14.28	20.78∗∗∗
		25	15853	6	3.78	4703	5	10.63	29.22∗∗∗

(a)The F₂ offspring is the number of nonsterile treated X-chromosomes
(b)MI: mutation frequency induced in 𝑚𝑢𝑠308⁻/mutation frequency induced in mus308 +
(c)Data from Sierra et al. [25]. One experiment was carried out mating the treated males to I,v;bw females to check the validity of these previous data for comparisons.
^∗𝑃<.05; ^∗∗𝑃<.01; ^∗∗∗𝑃<.001.