Mus308 Processes Oxygen and Nitrogen Ethylation DNA Damage in Germ Cells of Drosophila

Table 2

Molecular characterization of v mutants induced by DES in postmeiotic male germ cells, under 𝑚𝑢𝑠308⁻ conditions.

Mutant	Brood	Position	Mutation	Change	Sequence (5^-3^)^(a)
D8-4	F2-1B	92–556	464 bp deletion
D8-5	F2-1B	566	CG-TA	Leu-Phe	ACAG C TCCTG
D8-6	F2-1B	6	TA-AT	Ser-Arg	TCAG T TCGC
		129	TA-CG	intron	tcag t tctg
		323	TA-CG	intron	tgag t aggt
		398	CG-AT	Gln-Lys	CAAG C AGAT
		416	GC-TA	Asp-Tyr	GTTC G ACTC
D8-7	F2-1B	1167	GC-AT	Trp-STOP	AAGT G GAGA
D8-8	F2-2C	648	CG-TA	Ser-Phe	GCAT C TGGT
D8-9	F2-1A		No mutation
D8-11	F2-1C	−944	AT-TA		TATA A ATAT
		−243	TA-AT		TCAG T TATT
D8-12	F2-1A	492	GC-AT	Arg-Gln	AACC G AGTG
D8-14	F2-2A	494	GC-AT	Val-Met	CCGA G TGGT
D8-18	F2-2A	1128	TA-CG	Leu-Pro	TTGC T CACC
		1168	GC-AT	Trp-STOP	AGTG G AGAT
D8-19	F2-2A	596	AT-GC	Thr-Ala	GGAG A CCAT
D8-21	F2-2A	974	CG-TA	Arg-STOP	GAAG C GACG
D8-23	F2-2A	656	CG-TA	Gln-STOP	TTTT C AGTC
D8-26(b)	F2-2A	promotor
D8-27	F2-1C	322	GC-AT	intron	gtga g tag
D8-29	F2-2C	875	CG-TA	Gln-STOP	GTTT C AGGA
D8-30	F1-2B	974	CG-TA	Arg-STOP	GAAG C GACG
D8-31	F1-2C	−323	TA-AT		TCAG T TATT
		−945-935	10 bp deletion

(a)Since for some mutation types the damaged base could not be identified, the sequence surrounding the detected change in the coding strand is presented. Intron sequences are shown in lower case letters, exon sequences in capitals.
(b)See text for details.