Aflatoxin B₁-Associated DNA Adducts Stall S Phase and Stimulate Rad51 foci in Saccharomyces cerevisiae

Figure 3

Growth of wild type (a), rad51 (b), rad4 (e), and rad4 rad51 (d) cells after exposure to AFB₁. (Left) Growth of cells containing pCS316 and expressing CYP1A2 after chronic exposure to 0 μM (black), 25 μM (blue), and 50 μM (red) AFB₁. The relevant genotype is given below the panel (see Table 1, for complete genotype). 10⁵ log-phase cells were inoculated in each well, 𝑛=3. 𝐴₆₀₀ is plotted against time (h). (Right) rad4 cells synchronized in G1 were exposed to 50 μM AFB₁ for 3 hrs, washed, and inoculated on YPD. Cell growth was monitored from 0–18 hrs. Bars indicate the actual range of measurements.

(a)

(b)

(c)

(d)

(e)

(f)