Journal of Nucleic Acids

Review Article

Polymorphic Nucleic Acid Binding of Bioactive Isoquinoline Alkaloids and Their Role in Cancer

Table 3

Thermodynamic parameters for the interaction of various alkaloids with calf thymus DNA complexation obtained from isothermal titration calorimetric studiesa.
Alkaloid 𝐾 ( × 105 M−1) 𝑛 Δ 𝐺 ° (kcal mol−1) Δ 𝐻 ° (kcal mol−1) Δ 𝑆 ° (cal ⋅ deg−1 mol−1)References
Berberine 0 . 1 2 3 . 6 − 5 . 5 0 − 2 . 0 1 + 1 1 . 4 0 [28, 42]
Palmatine 0 . 2 8 3 . 0 − 5 . 8 2 − 4 . 4 0 + 5 . 0 0 [28, 56]
Coralyne 7 1 . 5 4 . 0 − 9 . 2 5 − 7 . 0 5 + 7 . 7 0 [28, 42]
Sanguinarine 9 . 5 2 2 . 2 − 8 . 0 2 − 6 . 9 1 + 3 . 7 0 [57]
aAverage of four determinations, 𝐾 and Δ 𝐻 ° values were determined from fits of the ITC profiles to Origin 7.0. The values of Δ 𝐺 ° and Δ 𝑆 ° were determined using the equations Δ 𝐺 ∘ = − ( 𝑅 𝑇 l n 𝐾 ) and Δ 𝐺 ∘ = Δ 𝐻 ∘ − 𝑇 Δ 𝑆 ∘ . respectively. The ITC profile was fit to a model of single binding sites, where 𝑛 denotes the binding site size.