Structural Biology of DNA Repair: Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining

Figure 14

SAXS study of LmX4 and Bm4. (a) SAXS data of LmX4 (green) and BmX4 (gold) where 𝑞=(4𝜋sin𝜃)/𝜆. (b) Guinier plots of LmX4 and BmX4 with liner fits (red line) 𝑞<1.3/Rg. (c) Shape reconstructions of BmX4 (gold) and LmX4 (green) created by using Dammin [130]. Ten individual models were calculated and averaged by Damaver [131]. NSD of BmX4 and LmX4 are 0.780 and 0.814, respectively. Molecular envelopes were produced using the UCSF Chimera package from the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIH P41 RR-01081) [132]. The crystallographic model of BmX4 (PDB code: 3II6) was superimposed into its molecular envelope by using Chimera.