Structural Biology of DNA Repair: Spatial Organisation of the Multicomponent Complexes of Nonhomologous End Joining
Figure 7
Schematic diagram of DNA-PKcs sequence-function implications. Shown are four highly conserved regions (HCR) [49]; Kinase, FAT, and FATC domains; putative sites for nuclear localization signals (NLSs) [49]; Autophosphorylation sites PQR [50], ABCDE [19], S3205 [51], S3821, S4026, T4102 [52], and T3950 [53]; cleavage sites for apoptotic protease caspase-3 [49, 54–56]; binding sites for Lyn tyrosine kinase [57], C1D protein that interacts at the leucine-zipper motif of DNA-PKcs [58], protein phosphatase-5 (PP5) that binds, using its tetratricopeptide repeat (TPR), to dephosphorylate DNA-PKcs at S2056 and T2609 [59], Ku70/80 that binds to present the damaged DNA double strand ends to DNA-PKcs [60], the kinase interacting protein (KIP) [61], and c-Abl that binds using its SH3 a binding that is triggered by DNA damage [60].
