Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of O⁶-Alkylguanine-DNA Alkyltransferase Activity

Table 1

Sequences of TBA oligonucleotide derivatives used in the development of the hAGT fluorescence assay. ^MeG represents O⁶-methylguanine. MB represents the fluorophore group (FAM), labelled in the 5^ end, and the quencher group (Dabsyl), labelled in the 3^ end of the sequence.

Abbreviation	Sequence
TBA	5^-GGT TGG TGT GGT TGG-3^
5-O^6-MeG-TBA	5^-GGT T^𝐌𝐞𝐆G TGT GGT TGG-3^
6-O^6-MeG-TBA	5^-GGT TG ^𝐌𝐞𝐆TGT GGT TGG-3^
C-TBA	5^-CCA ACC ACA CCA ACC-3^
MB-TBA	5^-FAM-GGT TGG TGT GGT TGG-Dabsyl-3^
MB-5-O^6-MeG-TBA	5^-FAM-GGT T ^𝐌𝐞𝐆G TGT GGT TGG- Dabsyl-3^
MB-6-O^6-MeG-TBA	5^-FAM-GGT TG ^𝐌𝐞𝐆 TGT GGT TGG- Dabsyl-3^
3-HP-TBA	5^-A CCT TTT GGT TGG TGT GGT TGG-3^
6-HP-TBA	5^-C CAA CCT TTT GGT TGG TGT GGT TGG-3^
9-HP-TBA	5^-A CAC CAA CCT TTT GGT TGG TGT GGT TGG-3^