Journal of Nucleic Acids / 2010 / Article / Tab 1

Research Article

Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of -Alkylguanine-DNA Alkyltransferase Activity

Table 1

Sequences of TBA oligonucleotide derivatives used in the development of the hAGT fluorescence assay. MeG represents O6-methylguanine. MB represents the fluorophore group (FAM), labelled in the 5 end, and the quencher group (Dabsyl), labelled in the 3 end of the sequence.

AbbreviationSequence

TBA 5 -GGT TGG TGT GGT TGG- 3
5-O6-MeG-TBA 5 -GGT T 𝐌 𝐞 𝐆 G TGT GGT TGG- 3
6-O6-MeG-TBA 5 -GGT TG 𝐌 𝐞 𝐆 TGT GGT TGG- 3
C-TBA 5 -CCA ACC ACA CCA ACC- 3
MB-TBA 5 -FAM-GGT TGG TGT GGT TGG-Dabsyl- 3
MB-5-O6-MeG-TBA 5 -FAM-GGT T 𝐌 𝐞 𝐆 G TGT GGT TGG- Dabsyl- 3
MB-6-O6-MeG-TBA 5 -FAM-GGT TG 𝐌 𝐞 𝐆 TGT GGT TGG- Dabsyl- 3
3-HP-TBA 5 -A CCT TTT GGT TGG TGT GGT TGG- 3
6-HP-TBA 5 -C CAA CCT TTT GGT TGG TGT GGT TGG- 3
9-HP-TBA 5 -A CAC CAA CCT TTT GGT TGG TGT GGT TGG- 3

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