Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana

Figure 3

TLS by ApPol𝜂 past CPD, 6–4 pp, and Dewar. Increasing amounts of ApPolη (12, 24, 59, 118, and 588 fmol in lanes 3–7, resp.) were incubated with 40 nM of the primer-templates indicated beside each panel. The templates contained undamaged TT (a), CPD (b), 6–4 pp (c), and Dewar (d). The samples for lanes 1 and 2 contained 0.1 U KF and no enzyme, respectively. The reactions were incubated at 37^∘C for 20 minutes, and then were subjected to 15% polyacrylamide/7.5 M urea gel electrophoresis. The autoradiograms of the gels are shown.