Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile <i>Alvinella pompejana</i>

<table>TLS by ApPol<i>η</i> past 5<i>R</i>-Tg, 5<i>S</i>-Tg, and 8-oxoG. Increasing amounts of ApPol<i>η</i> (12, 24, 59, 118, 588 fmol in lanes 3–7, resp.) were incubated with 40 nM of the primer-templates indicated beside each panel. The templates contained 5<i>R</i>-Tg (a), 5<i>S</i>-Tg (b), undamaged G (c), and 8-oxoG (d). The samples for lanes 1 and 2 contained 0.1 U T7 DNA polymerase and no enzyme, respectively. The reactions were incubated at 3<svg height="10.7375" id="M143" style="vertical-align:-0.10033pt" version="1.1" viewbox="0 0 12.3875 10.7375" width="12.3875" xmlns="http://www.w3.org/2000/svg">
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</svg>C for 20 minutes, and then were subjected to 15% polyacrylamide/7.5 M urea gel electrophoresis. The autoradiograms of the gels are shown.</table>

Journal of Nucleic Acids

fig5

Figure 5

Figure 5: Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile <i>Alvinella pompejana</i> 

Journal of Nucleic Acids

Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana

Figure 5