Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana

Figure 6

Selectivity of nucleotide incorporation by ApPol𝜂 opposite 5R-Tg (a), 5S-Tg (b), undamaged G (c), and 8-oxoG (d). ApPol𝜂 (12 fmol in lanes 2–7) was incubated with each primer-template in the presence of the four dNTPs (lane 3), the indicated dNTP (lanes 4–7), or the absence of dNTP (lane 2). Lane 1 contained no enzyme. The reactions were incubated at 37^∘C for 5 minutes, and then were subjected to 15% polyacrylamide/7.5 M urea gel electrophoresis. The autoradiograms of the gels are shown.