SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA
Figure 2
Phenomena appearing after depletion of SOD1. (a) Cell cycle analysis of cells. Cell cycle was analyzed using flow cytometry. Distribution patterns of asynchronous culture of Dox-treated cells for the indicated timepoints. The percentages of cells in each cell cycle phase and dead cells are shown on the right side of each panel. (b) Categorization of Giemsa-stained cells. Cells not having interphase nucleus, mitotic chromosomes, or hypercondensed chromosomes are classified into others in which apoptotic cells are included. Cells were harvested and treated with 75 mM KCl and fixed with methanol-acetic acid (3 : 1). The cell suspension was dropped onto wet glass slides, air dried, and stained with Giemsa solution (upper row). Percentages of cells exhibiting select phenomena were based on the categorization detailed in the boxes above the micrographs. At least 500 cells were counted in each sample. (c) Western blotting analysis of Lamin B1, with -actin used as loading control.
