SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA
Figure 3
Increases in superoxide, SCE frequency, and AP sites upon depletion of SOD1. (a) Superoxide level. Levels of superoxide were measured with BES-So-AM. The intensity of hSOD1 cells not treated with Dox was used as the standard (a.u.; arbitrary unit = 1). Error bars indicate standard errors based on three independent experiments. (b) SCE frequency. Schematic representation of cell treatment is depicted in upper panel. Cells were cultured for two cell cycles in a medium containing BrdU. Histograms represent SCEs in wild-type cells and in cells treated with Dox for the indicated timepoints. The mean and standard error are shown in the upper right corner. (c) AP sites. Scanning densitometric analysis of slot blots of endogenous AP sites was performed as described previously in [21]. Some cells were treated with Dox and/or APM for 112 hours. Bars indicate standard errors.
