SOD1 Is Essential for the Viability of DT40 Cells and Nuclear SOD1 Functions as a Guardian of Genomic DNA
Figure 5
Exclusion of SOD1 from nuclei increases SCE. (a) Distribution of SOD1 with NLS or NES localization signal. Upper panels show the subcellular localization of GFP-hSOD1 with and without localization signals. The middle and lower panels indicate Hoechst staining and merged images, respectively. (b) Growth curves of hSOD1 cells complemented with indicated hSOD1. Cells were cultured in the presence or absence of 1 g/mL Dox. (c) SCE frequency. SCE frequencies of hSOD1 cells expressing the indicated hSOD1 or those of hSOD2 cells cultured in the presence or absence of 1 g/mL Dox for 120 hours were measured. Bars indicate standard errors. *; student t-test, . (d) SCE frequency in the presence of paraquat. Paraquat was added at a final concentration of 100 M to the culture medium 20 hours prior to harvesting. SCE frequencies of hSOD1 cells expressing the indicated hSOD1 in the presence or absence of 1 g/mL Dox for 120 hours were measured. Bars indicate standard errors. *; student t-test, .
