Stimulation of DNA Glycosylase Activities by XPC Protein Complex: Roles of Protein-Protein Interactions
Figure 2
XPC stimulates the activity of TDG that cleaves uracil from G/U mismatches. The activity of TDG was measured by using 1.6 nM of 60-bp DNA containing a single G/U mismatch as a substrate. The reaction was done at C for the indicated time with 0.42 nM His-TDG in the presence of various concentrations of XPC-RAD23B. The DNA samples were then purified and subjected to alkali-treatment to cleave the resulting AP sites and separated with denaturing PAGE. The ratio of the cleaved product was calculated and plotted as a graph. The mean values and standard errors were calculated from at least two independent experiments.
