Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding

Table 2

Thermodynamic parameters obtained from CD and UV melting curves. UV melting curves were obtained at ~293 nm in the presence of 50 mM KCl. Values in which a two-state mechanism was clearly confirmed by dual-wavelength parametric test are highlighted by bold font.

Oligomer	Protrusion [nt.]	CD				UV
		𝑇𝑚	Δ𝐻	Δ𝑆	Δ𝐺^o37	𝑇𝑚	Δ𝐻	Δ𝑆	Δ𝐺^o37
		[^∘C]	[kcal⋅mol^−1]	[cal⋅mol^−1⋅K^−1]	[kcal⋅mol^−1]	[^∘C]	[kcal⋅mol^−1]	[cal⋅mol^−1⋅K^−1]	[kcal⋅ mol^−1]
TEL1	0–0	64.7	59.3	207	4.9	65.3	58.8	205	4.9
TEL2	0-3-0	55.9	39.5	135	2.3	55.3	34.0	116	1.9
5^-overhang
TEL1-5-TTA	3–0	57.6	57.9	198	3.6	57.8	58.7	201	3.7
TEL1-5-T-7	7–0	53.6	51.4	174	2.6	50.6	39.2	132	1.6
TEL1-5-A-7	7–0	54.8	39.0	133	2.1	56.0	41.8	143	2.4
TEL1-5-T-11	11–0	55.1	51.6	175	2.8	54.9	60.7	206	3.3
TEL1-5-TTA-11	11–0	54.8	50.6	172	2.7	53.9	58.1	197	3.0
TEL1-5-T-18	18–0	51.2	43.4	143	1.9	nd	nd	nd	nd
TEL1-5-TTA-18
293 nm	18–0	51.0	42.4	137	1.8	nd	nd	nd	nd
263 nm		52.3	42.8	144	2.0	nd	nd	nd	nd
3^-overhang
TEL1-3-TTA	0–3	59.6	55.9	193	3.8	58.9	61.6	212	4.1
TEL1-3-T-7	0–7	56.8	52.8	181	3.2	55.4	59.1	201	3.3
TEL1-3-A-7	0–7	59.8	50.5	174	3.5	59.3	50.7	175	3.4
TEL1-3-T-11	0–11	57.9	57.5	197	3.6	56.1	26.8	92	1.6
TEL1-3-TTA-11	0–11	57.7	55.5	190	3.5	57.1	46.8	160	2.8
TEL1-3-T-18	0–18	53.4	45.8	155	2.3	nd	Nd	nd	Nd
3^- and 5^-overhangs
TEL1-3-5-T-7	7–7	47.5	55.9	186	1.8	48.3	39.5	132	1.4
TEL1-3-5-A-7	7–7	51.3	46.8	158	2.1	52.6	52.4	179	3.1
TEL1-ran 263 nm	14–4	53.5	37.5	127	1.9	nd	Nd	nd	nd
TEL1-3-5-TTA-18
290 nm	18–18	40.5	49.5	161	0.5	nd	nd	nd	nd
265 nm		40.9	37.6	129	0.5	nd	nd	nd	nd