Coincident In Vitro Analysis of DNA-PK-Dependent and -Independent Nonhomologous End Joining

Figure 5

DNA end-joining fidelity reactions run in the presence or absence of 5% PEG. DNA DSB end joining was performed with each of the four different restriction enzyme linearized plasmid DNA substrates indicated to assess the effects of different DSB overhang configurations (see text) upon the fidelity of DSB repair occurring under DNA-PK-independent and -dependent reaction conditions. DSB repair fidelity is measured as the fraction of total DNA end-joining reaction products that are capable of being recut with the restriction enzyme that was originally used to linearize the end-joining substrate DNA. All reactions were run in triplicate. Bars represent the average restriction enzyme redigestion efficiency of the end-joining reaction products, which is reported as % fidelity.